quantikine colorimetric elisa kit  (R&D Systems)

Journal: PLOS ONE

Article Title: The neuroprotective effect of human primary astrocytes in multiple sclerosis: In vitro model

doi: 10.1371/journal.pone.0300203

Figure Lengend Snippet: List of forward and reverse primers used in qPCR experiment.

Article Snippet: Human IL-1β/IL-1F2 DuoSet, Human VEGF DuoSet, Human Total BDNF Quantikine ELISA kits (R&D Systems, USA) and Human Interleukin-6 ELISA Kit (SUNLONGBIOTECH, China) were used for this purpose, following their manufacturer’s protocol.

Techniques:

Journal: PLOS ONE

Article Title: The neuroprotective effect of human primary astrocytes in multiple sclerosis: In vitro model

doi: 10.1371/journal.pone.0300203

Figure Lengend Snippet: Astrocyte primary cell culture treated with 50% concentrations of both MS-CSF (n = 7) and Ctrl-CSF (n = 9) for 72 hrs. Control groups were only exposed to culture medial only. The quantitative expression of mRNA was evaluated to several neurotrophic factors; (A) GFAP (B) GDNF (C) BDNF and (D) VEGF. Data were analyzed using one-way ANOVA followed by Bonferroni’s post hoc test and expressed as mean ± SEM. Level of significance was considered as * for p < 0.05, and ** for p < 0.01.

Article Snippet: Human IL-1β/IL-1F2 DuoSet, Human VEGF DuoSet, Human Total BDNF Quantikine ELISA kits (R&D Systems, USA) and Human Interleukin-6 ELISA Kit (SUNLONGBIOTECH, China) were used for this purpose, following their manufacturer’s protocol.

Techniques: Cell Culture, Control, Expressing

Journal: PLOS ONE

Article Title: The neuroprotective effect of human primary astrocytes in multiple sclerosis: In vitro model

doi: 10.1371/journal.pone.0300203

Figure Lengend Snippet: Control cells kept untreated and only cultured with complete media. (A) 50% MS-CSF treatment significantly increased the amount of secreted VEGF compared to both Control untreated and Ctrl-CSF treated groups. In contrast, (B) BDNF level was significantly elevated in MS-CSF treated group, where it was not affected by Ctrl-CSF treatment, compared to control untreated group. Data were analyzed using one-way ANOVA followed by Bonferroni’s post hoc test and expressed as mean ± SEM. Level of significance was considered as * for p < 0.05, **p < 0.01, ***P<0.0025, ****P<0.0001.

Article Snippet: Human IL-1β/IL-1F2 DuoSet, Human VEGF DuoSet, Human Total BDNF Quantikine ELISA kits (R&D Systems, USA) and Human Interleukin-6 ELISA Kit (SUNLONGBIOTECH, China) were used for this purpose, following their manufacturer’s protocol.

Techniques: Control, Cell Culture